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microarray hybridisation chamber kit  (Agilent technologies)


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    Structured Review

    Agilent technologies microarray hybridisation chamber kit
    (A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
    Microarray Hybridisation Chamber Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridisation+kit/bio_rxiv__2020__12__17__423258-123-26-30
    Average 90 stars, based on 1 article reviews
    microarray hybridisation chamber kit - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Genotype-phenotype map of an RNA-ligand complex"

    Article Title: Genotype-phenotype map of an RNA-ligand complex

    Journal: bioRxiv

    doi: 10.1101/2020.12.17.423258

    (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
    Figure Legend Snippet: (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

    Techniques Used: Microarray, Fluorescence, Concentration Assay, Standard Deviation

    Related Articles

    Gene Expression:

    Article Title: The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling
    Article Snippet: .. The Agilent Gene Expression Hybridisation Kit (Agilent Technologies) was used following manufacturer’s protocol. cRNA was hybridised on a 8 × 60K microarray at 65°C for 17 hr using Agilent‘s recommended hybridisation chamber and oven. .. Finally, the microarray was washed once with the Agilent Gene Expression Wash Buffer one for one minute at ambient temperature followed by a second wash with preheated (37°C) Gene Expression Wash Buffer two for 1 min. Fluorescence signals on microarrays were detected by the SureScan Microarray Scanner G2600D (Agilent Technologies) at a resolution of 3 micron for SurePrint G3 Gene Expression Microarrays, generating a 20 bit TIFF file.

    Article Title: Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach
    Article Snippet: The RNA extracted from the leaf samples was prepared for microarray hybridisation at the Genomic Service of the IBMCP Institute (Instituto de Biología Molecular y Celular de Plantas) in Valencia (Spain) by Agilent technologies. cDNA synthesis and labelling on Agilent Tomato microarrays were carried out using the Agilent One Colour RNA Spike-in Kit and the Agilent Low Input Quick Amp Labeling Kit. .. Microarray hybridisation and washing were next performed with the Agilent Gene Expression Hybridization kit and Gene Expression Wash Buffers. .. Agilent microarray 4*44 k (Agilent G2519F) was selected for hybridisation (reference AMADID 22270 Tomato ).

    Article Title: Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression
    Article Snippet: RNA was purified with the RNeasy Mini Kit (Qiagen) and the amount and labeling efficiency were measured with NanoDrop. .. Hybridization was done using an Agilent Gene Expression Hybridisation Kit, scanning with an Agilent G2565CA Microarray Scanner System. .. Data were processed using Agilent Feature Extraction Software (10.7) with the single-color gene expression protocol, and raw data were analyzed with ChipInspector Software (Genomatix, Munich, Germany).

    Hybridization:

    Article Title: The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling
    Article Snippet: .. The Agilent Gene Expression Hybridisation Kit (Agilent Technologies) was used following manufacturer’s protocol. cRNA was hybridised on a 8 × 60K microarray at 65°C for 17 hr using Agilent‘s recommended hybridisation chamber and oven. .. Finally, the microarray was washed once with the Agilent Gene Expression Wash Buffer one for one minute at ambient temperature followed by a second wash with preheated (37°C) Gene Expression Wash Buffer two for 1 min. Fluorescence signals on microarrays were detected by the SureScan Microarray Scanner G2600D (Agilent Technologies) at a resolution of 3 micron for SurePrint G3 Gene Expression Microarrays, generating a 20 bit TIFF file.

    Article Title: Genotype-phenotype map of an RNA-ligand complex
    Article Snippet: .. 40 μl of the hybridisation mix was transferred into each of eight wells on a hybridisation gasket slide (Agilent, G2534-60015) during hybridisation assembly using the Agilent Microarray Hybridisation Chamber Kit (Agilent, Cat No. G2534A) and following the standard protocol. ..

    Article Title: A dominant mesomelic dysplasia associated with a 1.0-Mb microduplication of HOXD gene cluster at 2q31.1.
    Article Snippet: A three-generation family with four patients affected by a novel mesomelic dysplasia was investigated for genome-wide DNA copy number variation profiles.. This revealed a microduplication of a 1.0-Mb chromosomal segment at 2q31.1 spanning nine Homeo box D (HOXD) genes that co-segregated with the phenotype.. Quantitative PCR analysis of a gene within this duplicated region showed consistent results.

    Article Title: Preferential induction of the AhR gene battery in HepaRG cells after a single or repeated exposure to heterocyclic aromatic amines.
    Article Snippet: Abbreviations: 3-MC, 3-methylcholanthrene; AhR, A α-naphthoflavone; CYP, Cytochrome P450; DMSO, Dime FCS, Fetal calf serum; HAA, Heterocyclic aromatic am Analysis; MeIQx, 2-amino-3,8-dimethylimidazo[4,5-f]q Nrf2, NF-E2-related factor 2; PhIP, 2-amino-1-methyl-6S.E.M., Standard error of the mean; XRE, xenobiotic-res ⁎ Corresponding author.. Inserm UMR 991, Faculté de Biologiques, F-35043 Rennes Cedex, France.. Fax: +33 2 E-mail addresses: Julie.Dumont@pasteur-lille.fr (J.

    Article Title: Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach
    Article Snippet: The RNA extracted from the leaf samples was prepared for microarray hybridisation at the Genomic Service of the IBMCP Institute (Instituto de Biología Molecular y Celular de Plantas) in Valencia (Spain) by Agilent technologies. cDNA synthesis and labelling on Agilent Tomato microarrays were carried out using the Agilent One Colour RNA Spike-in Kit and the Agilent Low Input Quick Amp Labeling Kit. .. Microarray hybridisation and washing were next performed with the Agilent Gene Expression Hybridization kit and Gene Expression Wash Buffers. .. Agilent microarray 4*44 k (Agilent G2519F) was selected for hybridisation (reference AMADID 22270 Tomato ).

    Article Title: Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression
    Article Snippet: RNA was purified with the RNeasy Mini Kit (Qiagen) and the amount and labeling efficiency were measured with NanoDrop. .. Hybridization was done using an Agilent Gene Expression Hybridisation Kit, scanning with an Agilent G2565CA Microarray Scanner System. .. Data were processed using Agilent Feature Extraction Software (10.7) with the single-color gene expression protocol, and raw data were analyzed with ChipInspector Software (Genomatix, Munich, Germany).

    Microarray:

    Article Title: The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling
    Article Snippet: .. The Agilent Gene Expression Hybridisation Kit (Agilent Technologies) was used following manufacturer’s protocol. cRNA was hybridised on a 8 × 60K microarray at 65°C for 17 hr using Agilent‘s recommended hybridisation chamber and oven. .. Finally, the microarray was washed once with the Agilent Gene Expression Wash Buffer one for one minute at ambient temperature followed by a second wash with preheated (37°C) Gene Expression Wash Buffer two for 1 min. Fluorescence signals on microarrays were detected by the SureScan Microarray Scanner G2600D (Agilent Technologies) at a resolution of 3 micron for SurePrint G3 Gene Expression Microarrays, generating a 20 bit TIFF file.

    Article Title: Genotype-phenotype map of an RNA-ligand complex
    Article Snippet: .. 40 μl of the hybridisation mix was transferred into each of eight wells on a hybridisation gasket slide (Agilent, G2534-60015) during hybridisation assembly using the Agilent Microarray Hybridisation Chamber Kit (Agilent, Cat No. G2534A) and following the standard protocol. ..

    Article Title: A dominant mesomelic dysplasia associated with a 1.0-Mb microduplication of HOXD gene cluster at 2q31.1.
    Article Snippet: A three-generation family with four patients affected by a novel mesomelic dysplasia was investigated for genome-wide DNA copy number variation profiles.. This revealed a microduplication of a 1.0-Mb chromosomal segment at 2q31.1 spanning nine Homeo box D (HOXD) genes that co-segregated with the phenotype.. Quantitative PCR analysis of a gene within this duplicated region showed consistent results.

    Article Title: Preferential induction of the AhR gene battery in HepaRG cells after a single or repeated exposure to heterocyclic aromatic amines.
    Article Snippet: Abbreviations: 3-MC, 3-methylcholanthrene; AhR, A α-naphthoflavone; CYP, Cytochrome P450; DMSO, Dime FCS, Fetal calf serum; HAA, Heterocyclic aromatic am Analysis; MeIQx, 2-amino-3,8-dimethylimidazo[4,5-f]q Nrf2, NF-E2-related factor 2; PhIP, 2-amino-1-methyl-6S.E.M., Standard error of the mean; XRE, xenobiotic-res ⁎ Corresponding author.. Inserm UMR 991, Faculté de Biologiques, F-35043 Rennes Cedex, France.. Fax: +33 2 E-mail addresses: Julie.Dumont@pasteur-lille.fr (J.

    Article Title: Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach
    Article Snippet: The RNA extracted from the leaf samples was prepared for microarray hybridisation at the Genomic Service of the IBMCP Institute (Instituto de Biología Molecular y Celular de Plantas) in Valencia (Spain) by Agilent technologies. cDNA synthesis and labelling on Agilent Tomato microarrays were carried out using the Agilent One Colour RNA Spike-in Kit and the Agilent Low Input Quick Amp Labeling Kit. .. Microarray hybridisation and washing were next performed with the Agilent Gene Expression Hybridization kit and Gene Expression Wash Buffers. .. Agilent microarray 4*44 k (Agilent G2519F) was selected for hybridisation (reference AMADID 22270 Tomato ).

    Article Title: Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression
    Article Snippet: RNA was purified with the RNeasy Mini Kit (Qiagen) and the amount and labeling efficiency were measured with NanoDrop. .. Hybridization was done using an Agilent Gene Expression Hybridisation Kit, scanning with an Agilent G2565CA Microarray Scanner System. .. Data were processed using Agilent Feature Extraction Software (10.7) with the single-color gene expression protocol, and raw data were analyzed with ChipInspector Software (Genomatix, Munich, Germany).

    Genome Wide:

    Article Title: A dominant mesomelic dysplasia associated with a 1.0-Mb microduplication of HOXD gene cluster at 2q31.1.
    Article Snippet: A three-generation family with four patients affected by a novel mesomelic dysplasia was investigated for genome-wide DNA copy number variation profiles.. This revealed a microduplication of a 1.0-Mb chromosomal segment at 2q31.1 spanning nine Homeo box D (HOXD) genes that co-segregated with the phenotype.. Quantitative PCR analysis of a gene within this duplicated region showed consistent results.

    Control:

    Article Title: Preferential induction of the AhR gene battery in HepaRG cells after a single or repeated exposure to heterocyclic aromatic amines.
    Article Snippet: Abbreviations: 3-MC, 3-methylcholanthrene; AhR, A α-naphthoflavone; CYP, Cytochrome P450; DMSO, Dime FCS, Fetal calf serum; HAA, Heterocyclic aromatic am Analysis; MeIQx, 2-amino-3,8-dimethylimidazo[4,5-f]q Nrf2, NF-E2-related factor 2; PhIP, 2-amino-1-methyl-6S.E.M., Standard error of the mean; XRE, xenobiotic-res ⁎ Corresponding author.. Inserm UMR 991, Faculté de Biologiques, F-35043 Rennes Cedex, France.. Fax: +33 2 E-mail addresses: Julie.Dumont@pasteur-lille.fr (J.

    other:

    Article Title: Immunohistochemical expression of HER2 in breast cancer: socioeconomic impact of inaccurate tests
    Article Snippet: There are two shipments (or runs) per year, each of which contains a 5-core microarray slide of breast cancer cores with varying predefined HER2 expression (0/1+/2+/3+) and amplification levels (both amplified and unamplified for 2+ cancer cores) as verified by using two IHC FDA/CE-IVD approved assays (HercepTestTM, Dako, Glostrup, Denmark; PATHWAY®, Ventana Medical Systems, Inc., Tucson, AZ, USA) in NordiQC reference laboratories, and using the HER2 fluorescence in situ hybridisation (FISH) pharmDXTM Kit (Dako).

    Article Title: Amplification of PVT-1 is involved in poor prognosis via apoptosis inhibition in colorectal cancers
    Article Snippet: Caspase 3 and SMAD4 proteins were normalised to the level of β -tubulin protein (Cell Signaling Technology) diluted 1 : 1000.



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    (A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
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    Image Search Results


    (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

    Journal: bioRxiv

    Article Title: Genotype-phenotype map of an RNA-ligand complex

    doi: 10.1101/2020.12.17.423258

    Figure Lengend Snippet: (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

    Article Snippet: 40 μl of the hybridisation mix was transferred into each of eight wells on a hybridisation gasket slide (Agilent, G2534-60015) during hybridisation assembly using the Agilent Microarray Hybridisation Chamber Kit (Agilent, Cat No. G2534A) and following the standard protocol.

    Techniques: Microarray, Fluorescence, Concentration Assay, Standard Deviation